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Development of a counterselectable seamless mutagenesis system in lactic acid bacteria

BACKGROUND: Lactic acid bacteria (LAB) are receiving more attention to act as cell factories for the production of high-value metabolites. However, the molecular tools for genetic modifying these strains are mainly vector-based double-crossover strategies, which are laborious and inefficient. To add...

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Bibliographic Details
Published in:Microb Cell Fact
Main Authors: Xin, Yongping, Guo, Tingting, Mu, Yingli, Kong, Jian
Format: Artigo
Language:Inglês
Published: BioMed Central 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5499019/
https://ncbi.nlm.nih.gov/pubmed/28679374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-017-0731-8
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