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Effects of protein engineering and rational mutagenesis on crystal lattice of single chain antibody fragments

Protein crystallization is dependent upon, and sensitive to, the intermolecular contacts that assist in ordering proteins into a three dimensional lattice. Here we used protein engineering and mutagenesis to affect the crystallization of single chain antibody fragments (scFvs) that recognize the EE...

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Detalhes bibliográficos
Main Authors: Kalyoncu, Sibel, Hyun, Jeongmin, Pai, Jennifer C., Johnson, Jennifer L., Entzminger, Kevin, Jain, Avni, Heaner, David P., Morales, Ivan A., Truskett, Thomas M., Maynard, Jennifer A., Lieberman, Raquel L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4142072/
https://ncbi.nlm.nih.gov/pubmed/24615866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prot.24542
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