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Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy

We report the solution structure of Escherichia coli β-galactosidase (∼465 kDa), solved at ∼3.2-Å resolution by using single-particle cryo-electron microscopy (cryo-EM). Densities for most side chains, including those of residues in the active site, and a catalytic Mg(2+) ion can be discerned in the...

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Detalhes bibliográficos
Main Authors: Bartesaghi, Alberto, Matthies, Doreen, Banerjee, Soojay, Merk, Alan, Subramaniam, Sriram
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4136629/
https://ncbi.nlm.nih.gov/pubmed/25071206
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1402809111
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