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Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds
BACKGROUND: Urease, one of the highly efficient known enzymes, catalyzes the hydrolysis of urea into ammonia and carbon dioxide. The present study aimed to extract urease from pea seeds (Pisum Sativum L). The enzyme was then purified in three consequence steps: acetone precipitation, DEAE-cellulose...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
BioMed Central
2014
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4121304/ https://ncbi.nlm.nih.gov/pubmed/25065975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-15-15 |
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