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Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds

BACKGROUND: Urease, one of the highly efficient known enzymes, catalyzes the hydrolysis of urea into ammonia and carbon dioxide. The present study aimed to extract urease from pea seeds (Pisum Sativum L). The enzyme was then purified in three consequence steps: acetone precipitation, DEAE-cellulose...

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Detalhes bibliográficos
Main Authors: EL-Hefnawy, Mohamed E, Sakran, Mohamed, Ismail, Ali I, Aboelfetoh, Eman Fahmy
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4121304/
https://ncbi.nlm.nih.gov/pubmed/25065975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-15-15
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