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Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds
BACKGROUND: Urease, one of the highly efficient known enzymes, catalyzes the hydrolysis of urea into ammonia and carbon dioxide. The present study aimed to extract urease from pea seeds (Pisum Sativum L). The enzyme was then purified in three consequence steps: acetone precipitation, DEAE-cellulose...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4121304/ https://ncbi.nlm.nih.gov/pubmed/25065975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-15-15 |
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