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Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds

BACKGROUND: Urease, one of the highly efficient known enzymes, catalyzes the hydrolysis of urea into ammonia and carbon dioxide. The present study aimed to extract urease from pea seeds (Pisum Sativum L). The enzyme was then purified in three consequence steps: acetone precipitation, DEAE-cellulose...

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Bibliografski detalji
Glavni autori: EL-Hefnawy, Mohamed E, Sakran, Mohamed, Ismail, Ali I, Aboelfetoh, Eman Fahmy
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2014
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4121304/
https://ncbi.nlm.nih.gov/pubmed/25065975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-15-15
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