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Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds
BACKGROUND: Urease, one of the highly efficient known enzymes, catalyzes the hydrolysis of urea into ammonia and carbon dioxide. The present study aimed to extract urease from pea seeds (Pisum Sativum L). The enzyme was then purified in three consequence steps: acetone precipitation, DEAE-cellulose...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4121304/ https://ncbi.nlm.nih.gov/pubmed/25065975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-15-15 |
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