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A Quantitative Microscopy Technique for Determining the Number of Specific Proteins in Cellular Compartments
This protocol describes a method to determine both the average number and variance of proteins in the few to tens of copies in isolated cellular compartments, such as organelles and protein complexes. Other currently available protein quantification techniques either provide an average number but la...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4121127/ https://ncbi.nlm.nih.gov/pubmed/22094731 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.414 |
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