A Quantitative Microscopy Technique for Determining the Number of Specific Proteins in Cellular Compartments

This protocol describes a method to determine both the average number and variance of proteins in the few to tens of copies in isolated cellular compartments, such as organelles and protein complexes. Other currently available protein quantification techniques either provide an average number but la...

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Detaylı Bibliyografya
Asıl Yazarlar: Mutch, Sarah A., Gadd, Jennifer C., Fujimoto, Bryant S., Kensel-Hammes, Patricia, Schiro, Perry G., Bajjalieh, Sandra M., Chiu, Daniel T.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2011
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4121127/
https://ncbi.nlm.nih.gov/pubmed/22094731
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.414
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