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Deconvolving Single-Molecule Intensity Distributions for Quantitative Microscopy Measurements

In fluorescence microscopy, images often contain puncta in which the fluorescent molecules are spatially clustered. This article describes a method that uses single-molecule intensity distributions to deconvolve the number of fluorophores present in fluorescent puncta as a way to “count” protein num...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Mutch, Sarah A., Fujimoto, Bryant S., Kuyper, Christopher L., Kuo, Jason S., Bajjalieh, Sandra M., Chiu, Daniel T.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: The Biophysical Society 2007
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1831712/
https://ncbi.nlm.nih.gov/pubmed/17259276
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.101428
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