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Deconvolving Single-Molecule Intensity Distributions for Quantitative Microscopy Measurements
In fluorescence microscopy, images often contain puncta in which the fluorescent molecules are spatially clustered. This article describes a method that uses single-molecule intensity distributions to deconvolve the number of fluorophores present in fluorescent puncta as a way to “count” protein num...
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| Main Authors: | , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
The Biophysical Society
2007
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1831712/ https://ncbi.nlm.nih.gov/pubmed/17259276 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.101428 |
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