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Chemical Rescue of Enzymes: Proton Transfer in Mutants of Human Carbonic Anhydrase II
In human carbonic anhydrase II (HCA II) the mutation of position 64 from histidine to alanine (H64A) disrupts the rate limiting proton transfer (PT) event, resulting in a reduction of the catalytic activity of the enzyme as compared to the wild-type. Potential of mean force (PMF) calculations utiliz...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4120857/ https://ncbi.nlm.nih.gov/pubmed/21452838 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja1097594 |
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