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Engineering processive DNA polymerases with maximum benefit at minimum cost

DNA polymerases need to be engineered to achieve optimal performance for biotechnological applications, which often require high fidelity replication when using modified nucleotides and when replicating difficult DNA sequences. These tasks are achieved for the bacteriophage T4 DNA polymerase by repl...

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Autors principals: Reha-Krantz, Linda J., Woodgate, Sandra, Goodman, Myron F.
Format: Artigo
Idioma:Inglês
Publicat: Frontiers Media S.A. 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4120765/
https://ncbi.nlm.nih.gov/pubmed/25136334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2014.00380
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