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Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolution
The telomere repeat amplification protocol (TRAP) for the human reverse transcriptase, telomerase, is a PCR-based assay developed two decades ago and is still used for routine determination of telomerase activity. The TRAP assay can only reproducibly detect ∼2-fold differences and is only quantitati...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4117742/ https://ncbi.nlm.nih.gov/pubmed/24861623 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku439 |
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