3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells

Synthetic 3′-biotin-tagged microRNAs (miRNAs) have often been used to select interacting messenger RNA (mRNA) and noncoding RNA (ncRNA) targets. Here, we examined the extent of association of 3′-end biotinylated miR-27 with Argonaute (Ago) proteins in transfected human cells using a coimmunoprecipit...

Full description

Saved in:
Bibliographic Details
Main Authors: Guo, Yang Eric, Steitz, Joan A.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2014
Subjects:
Letter to the Editor
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4114695/
https://ncbi.nlm.nih.gov/pubmed/24821854
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.045054.114
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items