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3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells

Synthetic 3′-biotin-tagged microRNAs (miRNAs) have often been used to select interacting messenger RNA (mRNA) and noncoding RNA (ncRNA) targets. Here, we examined the extent of association of 3′-end biotinylated miR-27 with Argonaute (Ago) proteins in transfected human cells using a coimmunoprecipit...

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Detalhes bibliográficos
Main Authors: Guo, Yang Eric, Steitz, Joan A.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4114695/
https://ncbi.nlm.nih.gov/pubmed/24821854
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.045054.114
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