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Engineering ribosomal leaky scanning and upstream open reading frames for precise control of protein translation

We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own appl...

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Autori principali: Ferreira, Joshua P, Noderer, William L, Diaz de Arce, Alexander J, Wang, Clifford L
Natura: Artigo
Lingua:Inglês
Pubblicazione: Landes Bioscience 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4101011/
https://ncbi.nlm.nih.gov/pubmed/24637490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/bioe.27607
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