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Tuning gene expression with synthetic upstream open reading frames
We engineered short ORFs and used them to control the expression level of recombinant proteins. These short ORFs, encoding a two-amino acid peptide, were placed upstream of an ORF encoding a protein of interest. Insertion of these upstream ORFs (uORFs) resulted in suppression of protein expression....
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3710870/ https://ncbi.nlm.nih.gov/pubmed/23798422 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1305590110 |
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