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What’s in your buffer? Solute altered millisecond motions detected by solution NMR

To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate, in comparison to MES- and HEPES-buffered references, on the chemical...

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Autors principals: Wong, Madeline, Khirich, Gennady, Loria, J. Patrick
Format: Artigo
Idioma:Inglês
Publicat: 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4096712/
https://ncbi.nlm.nih.gov/pubmed/23991940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi400973e
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