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What’s in your buffer? Solute altered millisecond motions detected by solution NMR

To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate, in comparison to MES- and HEPES-buffered references, on the chemical...

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Main Authors: Wong, Madeline, Khirich, Gennady, Loria, J. Patrick
Formato: Artigo
Idioma:Inglês
Publicado: 2013
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4096712/
https://ncbi.nlm.nih.gov/pubmed/23991940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi400973e
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