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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
We describe the use of Bio-layer Interferometry to study inhibitory interactions of subunit ε with the catalytic complex of Escherichia coli ATP synthase. Bacterial F-type ATP synthaseis the target of a new, FDA-approved antibiotic to combat drug-resistant tuberculosis. Understanding bacteria-specif...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4089413/ https://ncbi.nlm.nih.gov/pubmed/24638157 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51383 |
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