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Off-target effect of doublecortin-family shRNA on neuronal migration associated with endogenous microRNA dysregulation

Acute gene inactivation using short hairpin RNA (shRNA, knockdown) in developing brain is a powerful technique to study genetic function, however, discrepancies between knockdown and knockout murine phenotypes have left unanswered questions. For example, doublecortin (Dcx) knockdown but not knockout...

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Detalhes bibliográficos
Main Authors: Baek, Seung Tae, Kerjan, Geraldine, Bielas, Stephanie L., Lee, Ji Eun, Fenstermaker, Ali G., Novarino, Gaia, Gleeson, Joseph G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4086250/
https://ncbi.nlm.nih.gov/pubmed/24945770
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.neuron.2014.04.036
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