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TR-FRET Assays of Huntingtin Protein Fragments Reveal Temperature and PolyQ Length-Dependent Conformational Changes

Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology is a widely used immunoassay that enables high-throughput quantitative measurements of proteins of interest. One of the well established examples is the TR-FRET assay for mutant huntingtin protein (HTT), which is the major cau...

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Bibliografiska uppgifter
Huvudupphovsmän: Cui, Xiaotian, Liang, Qingnan, Liang, Yijian, Lu, Mingxing, Ding, Yu, Lu, Boxun
Materialtyp: Artigo
Språk:Inglês
Publicerad: Nature Publishing Group 2014
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4083280/
https://ncbi.nlm.nih.gov/pubmed/24998512
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep05601
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