Direct isolation of poly(A)(+) RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture

LNA oligonucleotides constitute a class of bicyclic RNA analogues having an exceptionally high affinity for their complementary DNA and RNA target molecules. We here report a novel method for highly efficient isolation of intact poly(A)(+) RNA using an LNA-substituted oligo(dT) affinity ligand, base...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Jacobsen, Nana, Nielsen, Peter Stein, Jeffares, Daniel Charlton, Eriksen, Jens, Ohlsson, Helle, Arctander, Peter, Kauppinen, Sakari
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2004
Pynciau:
NAR Methods Online
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC407836/
https://ncbi.nlm.nih.gov/pubmed/15096560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh056
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