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Distinct Effects of Guanidine Thiocyanate on the Structure of Superfolder GFP

Having a high folding efficiency and a low tendency to aggregate, the superfolder GFP (sfGFP) offers a unique opportunity to study the folding of proteins that have a β-barrel topology. Here, we studied the unfolding–refolding of sfGFP that was induced by guanidine thiocyanate (GTC), which is a stro...

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Detalhes bibliográficos
Main Authors: Stepanenko, Olesya V., Stepanenko, Olga V., Kuznetsova, Irina M., Shcherbakova, Daria M., Verkhusha, Vladislav V., Turoverov, Konstantin K.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3492234/
https://ncbi.nlm.nih.gov/pubmed/23144981
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0048809
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