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Direct isolation of poly(A)(+) RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture
LNA oligonucleotides constitute a class of bicyclic RNA analogues having an exceptionally high affinity for their complementary DNA and RNA target molecules. We here report a novel method for highly efficient isolation of intact poly(A)(+) RNA using an LNA-substituted oligo(dT) affinity ligand, base...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC407836/ https://ncbi.nlm.nih.gov/pubmed/15096560 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh056 |
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