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Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics()

Trypsin is an endoprotease commonly used for sample preparation in proteomics experiments. Importantly, protein digestion is dependent on multiple factors, including the trypsin origin and digestion conditions. In-depth characterization of trypsin activity could lead to improved reliability of pepti...

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Main Authors: Walmsley, Scott J., Rudnick, Paul A., Liang, Yuxue, Dong, Qian, Stein, Stephen E., Nesvizhskii, Alexey I.
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4076643/
https://ncbi.nlm.nih.gov/pubmed/24116745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr400611h
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