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Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin

[Image: see text] Trypsin is the most used enzyme in proteomics. Nevertheless, proteases with complementary cleavage specificity have been applied in special circumstances. In this work, we analyzed the characteristics of five protease alternatives to trypsin for protein identification and sequence...

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Библиографические подробности
Опубликовано в: :Anal Chem
Главные авторы: Dau, Therese, Bartolomucci, Giulia, Rappsilber, Juri
Формат: Artigo
Язык:Inglês
Опубликовано: American Chemical Society 2020
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC7377536/
https://ncbi.nlm.nih.gov/pubmed/32628831
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.0c00478
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