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Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin

[Image: see text] Trypsin is the most used enzyme in proteomics. Nevertheless, proteases with complementary cleavage specificity have been applied in special circumstances. In this work, we analyzed the characteristics of five protease alternatives to trypsin for protein identification and sequence...

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Bibliografske podrobnosti
izdano v:Anal Chem
Main Authors: Dau, Therese, Bartolomucci, Giulia, Rappsilber, Juri
Format: Artigo
Jezik:Inglês
Izdano: American Chemical Society 2020
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7377536/
https://ncbi.nlm.nih.gov/pubmed/32628831
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.0c00478
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