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Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin

[Image: see text] Trypsin is the most used enzyme in proteomics. Nevertheless, proteases with complementary cleavage specificity have been applied in special circumstances. In this work, we analyzed the characteristics of five protease alternatives to trypsin for protein identification and sequence...

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Bibliografiske detaljer
Udgivet i:Anal Chem
Main Authors: Dau, Therese, Bartolomucci, Giulia, Rappsilber, Juri
Format: Artigo
Sprog:Inglês
Udgivet: American Chemical Society 2020
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7377536/
https://ncbi.nlm.nih.gov/pubmed/32628831
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.0c00478
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