Utilization of the AAVS1 Safe Harbor Locus for Hematopoietic Specific Transgene Expression and Gene Knockdown in Human ES Cells

Human pluripotent stem cells offer a powerful system to study human biology and disease. Here, we report a system to both express transgenes specifically in ES cell derived hematopoietic cells and knockdown gene expression stably throughout the differentiation of ES cells. We characterize a CD43 pro...

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Detalhes bibliográficos
Main Authors: Tiyaboonchai, Amita, Mac, Helen, Shamsedeen, Razveen, Mills, Jason A., Kishore, Siddarth, French, Deborah L., Gadue, Paul
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4048956/
https://ncbi.nlm.nih.gov/pubmed/24631742
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.scr.2014.02.004
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