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A PCR Based Protocol for Detecting Indel Mutations Induced by TALENs and CRISPR/Cas9 in Zebrafish

Genome editing techniques such as the zinc-finger nucleases (ZFNs), transcription activator-like effecter nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system Cas9 can induce efficient DNA double strand breaks (DSBs) at the target g...

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Detalhes bibliográficos
Main Authors: Yu, Chuan, Zhang, Yaguang, Yao, Shaohua, Wei, Yuquan
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4046980/
https://ncbi.nlm.nih.gov/pubmed/24901507
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0098282
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