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Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella
A real-time, genus-specific 5′ nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did n...
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| Auteurs principaux: | , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology
2004
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC404622/ https://ncbi.nlm.nih.gov/pubmed/15131207 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.5.2261-2263.2004 |
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