Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella

A real-time, genus-specific 5′ nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did n...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Bogdanovich, T., Skurnik, M., Lübeck, P. S., Ahrens, P., Hoorfar, J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2004
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC404622/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15131207/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.42.5.2261-2263.2004
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