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Demonstration of a low-energy on-pathway intermediate in a fast-folding protein by kinetics, protein engineering, and simulation

It is controversial whether fast-folding proteins can form productive on-pathway intermediates that are more stable than the denatured state because noncovalent intermediates are usually evanescent. Here, we apply the classical criteria for the existence of intermediates: namely, the intermediates f...

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Hlavní autoři: Jemth, Per, Gianni, Stefano, Day, Ryan, Li, Bin, Johnson, Christopher M., Daggett, Valerie, Fersht, Alan R.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2004
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC404065/
https://ncbi.nlm.nih.gov/pubmed/15096617
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401732101
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