Single-molecule assay reveals strand switching and enhanced processivity of UvrD

DNA helicases are enzymes capable of unwinding double-stranded DNA (dsDNA) to provide the single-stranded DNA template required in many biological processes. Among these, UvrD, an essential DNA repair enzyme, has been shown to unwind dsDNA while moving 3′-5′ on one strand. Here, we use a single-mole...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Autores principales: Dessinges, Marie-Noëlle, Lionnet, Timothée, Xi, Xu Guang, Bensimon, David, Croquette, Vincent
Formato: Artigo
Lenguaje:Inglês
Publicado: National Academy of Sciences 2004
Materias:
Biological Sciences
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC404063/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15079074/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0306713101
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