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A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering
A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique – USER cloning – to rapidly construct mammalian expression vectors of multiple DNA fragments...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4039435/ https://ncbi.nlm.nih.gov/pubmed/24879460 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0096693 |
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