A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering

A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique – USER cloning – to rapidly construct mammalian expression vectors of multiple DNA fragments...

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Bibliografiske detaljer
Main Authors: Lund, Anne Mathilde, Kildegaard, Helene Faustrup, Petersen, Maja Borup Kjær, Rank, Julie, Hansen, Bjarne Gram, Andersen, Mikael Rørdam, Mortensen, Uffe Hasbro
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2014
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4039435/
https://ncbi.nlm.nih.gov/pubmed/24879460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0096693
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