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Isolation and accumulation of spliceosomal assembly intermediates
Isolating spliceosomes at a specific assembly stage requires a means to stall or enrich for one of the intermediate splicing complexes. We describe strategies to arrest spliceosomes at different points of complex formation and provide a detailed protocol developed for isolating intact splicing compl...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2014
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4038131/ https://ncbi.nlm.nih.gov/pubmed/24549665 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-980-2_14 |
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