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Isolation and accumulation of spliceosomal assembly intermediates

Isolating spliceosomes at a specific assembly stage requires a means to stall or enrich for one of the intermediate splicing complexes. We describe strategies to arrest spliceosomes at different points of complex formation and provide a detailed protocol developed for isolating intact splicing compl...

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Detaylı Bibliyografya
Asıl Yazarlar: Ilagan, Janine O., Jurica, Melissa S.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2014
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4038131/
https://ncbi.nlm.nih.gov/pubmed/24549665
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-980-2_14
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