Systematic Cloning of Treponema pallidum Open Reading Frames for Protein Expression and Antigen Discovery

A topoisomerase-based method was used to clone PCR products encoding 991 of the 1041 open reading frames identified in the genome sequence of the bacterium that causes syphilis, Treponema pallidum subsp. pallidum. Cloning the open reading frames into the univector plasmid system permitted the rapid...

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Dettagli Bibliografici
Pubblicato in:Genome Res
Autori principali: McKevitt, Matthew, Patel, Krupa, Smajs, David, Marsh, Michael, McLoughlin, Melanie, Norris, Steven J., Weinstock, George M., Palzkill, Timothy
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2003
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Letters
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC403740/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12805273/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.288103
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