Systematic Cloning of Treponema pallidum Open Reading Frames for Protein Expression and Antigen Discovery

A topoisomerase-based method was used to clone PCR products encoding 991 of the 1041 open reading frames identified in the genome sequence of the bacterium that causes syphilis, Treponema pallidum subsp. pallidum. Cloning the open reading frames into the univector plasmid system permitted the rapid...

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Hauptverfasser: McKevitt, Matthew, Patel, Krupa, Smajs, David, Marsh, Michael, McLoughlin, Melanie, Norris, Steven J., Weinstock, George M., Palzkill, Timothy
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2003
Schlagworte:
Letters
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC403740/
https://ncbi.nlm.nih.gov/pubmed/12805273
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.288103
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