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Systematic Cloning of Treponema pallidum Open Reading Frames for Protein Expression and Antigen Discovery

A topoisomerase-based method was used to clone PCR products encoding 991 of the 1041 open reading frames identified in the genome sequence of the bacterium that causes syphilis, Treponema pallidum subsp. pallidum. Cloning the open reading frames into the univector plasmid system permitted the rapid...

詳細記述

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書誌詳細
出版年:Genome Res
主要な著者: McKevitt, Matthew, Patel, Krupa, Smajs, David, Marsh, Michael, McLoughlin, Melanie, Norris, Steven J., Weinstock, George M., Palzkill, Timothy
フォーマット: Artigo
言語:Inglês
出版事項: Cold Spring Harbor Laboratory Press 2003
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC403740/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12805273/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.288103
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