Systematic Cloning of Treponema pallidum Open Reading Frames for Protein Expression and Antigen Discovery

A topoisomerase-based method was used to clone PCR products encoding 991 of the 1041 open reading frames identified in the genome sequence of the bacterium that causes syphilis, Treponema pallidum subsp. pallidum. Cloning the open reading frames into the univector plasmid system permitted the rapid...

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Main Authors: McKevitt, Matthew, Patel, Krupa, Smajs, David, Marsh, Michael, McLoughlin, Melanie, Norris, Steven J., Weinstock, George M., Palzkill, Timothy
格式: Artigo
語言:Inglês
出版: Cold Spring Harbor Laboratory Press 2003
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Letters
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC403740/
https://ncbi.nlm.nih.gov/pubmed/12805273
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.288103
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