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Ubiquitin Ligase Trapping Identifies an SCF(Saf1) Pathway Targeting Unprocessed Vacuolar/Lysosomal Proteins
We have developed a technique, called Ubiquitin Ligase Substrate Trapping, for the isolation of ubiquitinated substrates in complex with their ubiquitin ligase (E3). By fusing a ubiquitin associated (UBA) domain to an E3 ligase, we were able to selectively purify the polyubiquitinated forms of E3 su...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4032118/ https://ncbi.nlm.nih.gov/pubmed/24389104 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2013.12.003 |
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