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Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps)
Ubiquitination is an essential protein modification that influences eukaryotic processes ranging from substrate degradation to nonproteolytic pathway alterations, including DNA repair and endocytosis. Previous attempts to analyze substrates via affinity purification approach in which ubiquitin ligas...
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| Publicado no: | Nat Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6499077/ https://ncbi.nlm.nih.gov/pubmed/26766115 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.008 |
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