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Derivation and characterization of Dicer- and microRNA-deficient human cells
We have used genome editing to generate inactivating deletion mutations in all three copies of the dicer (hdcr) gene present in the human cell line 293T. As previously shown in murine ES cells lacking Dicer function, hDcr-deficient 293T cells are severely impaired for the production of mature microR...
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| 主要な著者: | , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Cold Spring Harbor Laboratory Press
2014
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4024645/ https://ncbi.nlm.nih.gov/pubmed/24757167 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.044545.114 |
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