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Derivation and characterization of Dicer- and microRNA-deficient human cells
We have used genome editing to generate inactivating deletion mutations in all three copies of the dicer (hdcr) gene present in the human cell line 293T. As previously shown in murine ES cells lacking Dicer function, hDcr-deficient 293T cells are severely impaired for the production of mature microR...
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| Main Authors: | , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Cold Spring Harbor Laboratory Press
2014
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4024645/ https://ncbi.nlm.nih.gov/pubmed/24757167 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.044545.114 |
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