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Broad Specificity Profiling of TALENs Results in Engineered Nucleases With Improved DNA Cleavage Specificity

Although transcription activator-like effector nucleases (TALENs) can be designed to cleave chosen DNA sequences, TALENs have been shown to have activity against related off-target sequences. To better understand TALEN specificity and engineer TALENs with improved specificity, we profiled 30 unique...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Guilinger, John P., Pattanayak, Vikram, Reyon, Deepak, Tsai, Shengdar Q., Sander, Jeffry D., Joung, J. Keith, Liu, David R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4010127/
https://ncbi.nlm.nih.gov/pubmed/24531420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2845
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