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In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors

Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active mole...

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Autors principals: Hoang, Thi-My-Nhung, Vu, Hong-Lien, Le, Ly-Thuy-Tram, Nguyen, Chi-Hung, Molla, Annie
Format: Artigo
Idioma:Inglês
Publicat: MDPI 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4009756/
https://ncbi.nlm.nih.gov/pubmed/24833340
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/biology3010167
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