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Investigating the Reactivities of a Polyketide Synthase Module through Fluorescent Click Chemistry
A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with sulforhodamine B azide.
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3999232/ https://ncbi.nlm.nih.gov/pubmed/24196586 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c3cc47513a |
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