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A Confirmation of the Quench-Cryoannealing Relaxation Protocol for Identifying Reduction States of Freeze-Trapped Nitrogenase Intermediates
[Image: see text] We have advanced a mechanism for nitrogenase catalysis that rests on the identification of a low-spin EPR signal (S = 1/2) trapped during turnover of a MoFe protein as the E(4) state, which has accumulated four reducing equivalents as two [Fe–H–Fe] bridging hydrides. Because electr...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American
Chemical Society
2014
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3993915/ https://ncbi.nlm.nih.gov/pubmed/24635454 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ic500013c |
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