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Visualization of intermediate and transition-state structures in protein-tyrosine phosphatase catalysis.

Engineering site-specific amino acid substitutions into the protein-tyrosine phosphatase (PTPase) PTP1 and the dual-specific vaccinia H1-related phosphatase (VHR), has kinetically isolated the two chemical steps of the reaction and provided a rare opportunity for examining transition states and dire...

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Autors principals: Denu, J M, Lohse, D L, Vijayalakshmi, J, Saper, M A, Dixon, J E
Format: Artigo
Idioma:Inglês
Publicat: 1996
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC39825/
https://ncbi.nlm.nih.gov/pubmed/8637902
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