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Optimizing the transient transfection process of HEK-293 suspension cells for protein production by nucleotide ratio monitoring
Large scale, transient gene expression (TGE) is highly dependent of the physiological status of a cell line. Therefore, intracellular nucleotide pools and ratios were used for identifying and monitoring the optimal status of a suspension cell line used for TGE. The transfection efficiency upon polye...
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| Autores principales: | , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Springer Netherlands
2013
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3973801/ https://ncbi.nlm.nih.gov/pubmed/23775287 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-013-9601-3 |
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